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technique histo tat


SJr
Go to solution Solved by Sashounet,

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@SJr Je confirme, la paraffine (apolaire) ne pourrait pas être incluse si il n'y a pas d'étape de déshydratation du tissu au préalable..

Recap des étapes : - fixation

-inclusion (de paraffaine, necessite une déshydratation)

- coupe

- coloration (necessite une réhydratation car les colorants sont polaires, soluble dans l'eau) puis déshydratation de nouveau pour conserver la coupe du tissu (un peu comme un taxidermiste)

- montage

 

Edited by Sashounet
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