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ccb 2018 2019 biomol


MaHa_logène
Go to solution Solved by ValentineMartel,

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  • Solution

Salut, 

 

Normalement oui, vous l'avez vu. La papaïne permet de couper un anticorps au niveau de ce qu'on appelle la charnière, c'est à dire la limite entre fragmente Fc et fragment Fab. 

image.png.d9d592c11a470bb3aea9e00c590278ba.png

Sur cette image, c'est la réaction de droite. On obtient donc 3 fragments  : 

  • 2 demi-chaines lourdes = 50 kDa (2x25)
  • 1 demi-chaine lourde + 1 chaine légère = 50 kDa (25 + 25)
  • 1 demi-chaine lourde + 1 chaine légère = 50 kDa (25 + 25)

 

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